Paper Title
The Effect of Combined Drug Treatment on Hl60 Leukemia Cell Survival : Drug Repurposing

Abstract
Drug discovery and development efforts generally do not provide a new drug into the market but deliver the scientific information to be used in the development of diagnostics and therapeutics. Generally drugs highly metabolized by detoxification system enzymes, such as Glutathione Transferases (GST), are of the limited use in clinics. In that sense the aim of the work is to evaluate the novel effect of Doxazosin, the drug with antihypertensive effect, in combination with anticancer drugs on HL60 cells. The study was also designed to evaluate the effect of combined drug use against the GST target. The results revealed that the treatment of cells with 5 nM SU6656 resulting in GST activation was reversed in the presence of Doxazosin with almost 50% inhibition of that enzyme. On the other hand Genistein alone resulting 57% GST inhibition was not significantly lowered in the presence of Doxazosin. Therefore it may be suggested that use of doxazosin with isoflavonoid or indolinone derivative drugs may enhance drug effectiveness. Keywords- doxazosin, SU6656, Genistein, Glutathione Transferase, Cytotoxicity, combined therapy, drug discovery.