Comparison Of Anti-Inflammatory Activities And Cell Viability In Herbal Medical Plants
In the situation that there are growing interest in repiratory diseases, such as allergic bronchial inflammation caused by recently rapid environmental change and deterioration of air. It is necessary to find functional materials that can used in aleviation of asthma, one of the respiratory disease of mordern people. Therefore, in the present study, 17 kinds of water extracts prepared from various herbal materials werecompared investigated the possibility of industrial application for treatment of respiratory disease using LPS-induced RAW 264.7 murine macrophage cells.At a dose of 500 μg/mL, with no cytotoxicity was observed in all extracts, Scutellaria baicalensis (Baikal skullcap),Combretumindicum(Rangoon crepper),and Cinnamon verum(Cinnamon) extracts showeddecreased levels of nitric oxide (NO) and IL-6, and IL-12 than those of other extracts by LPS-stimulated RAW 264.7 cells. These anti-inflammatory activities were dramatically increased when the dose treated were increased to 1000 μg/ml, however, some extracts, such as red ginseng root, S. baicalensis, and red ginseng root (85℃) showed the reduced viability of RAW 264.7, presuming to be cytotoxic effect. Meanwhile, C. verum,C. indicum, and Stephanomeriatenuifolia(narrow-leaved wire-lettuce)showed remarkable decrease of inflammatory mediators with no cytotoxicity in RAW 264.7 cells, compared with other extracts. Consequently, our results suggested that S. tenuifolia, C. indicum,C. verum extracts might be chosen for anti-inflammatory functional materials which could be useful to apply for the treatment of respiratory diseases.
Keywords: Herbal medicine, Anti-inflammatory, Cell viability, IL-6, IL-12