Paper Title
Effect Of Differentiation And Mineralizationin Mc3t3-E1 Cell treated By Protamexhydrolysate

Abstract
The purpose of this study was to determine which enzyme was the most suitable enzyme for Whey protein hydrolysis and the effect of hydrolyzed whey protein on bone differentiation and mineralization on MC3T3-E1 cell. First, we have determined through preliminary experiments that among the various enzymes, Proteomax is most suitable for hydrolyzing whey proteins. The whey hydrolyzate treated with Protamax was treated at different concentrations in the cells and Alkaline phosphatase (ALP) and calcium deposition rate were measured. The degree of gene expression related to growth was also measured. As results, ALP activity increased significantly (p<0.05) with a Protamex hydrolysate (PH) concentration of 6.25-50 mg/mL. The calcium depositioning increased as much as 161% at 50 mg/mL of PH compared to control. The gene expression levels of ALP and collagen type II (COL II) were significantly increased approximately 1.4-fold and 1.9-fold of control. PH increased significantly the mRNA level of bone sialoprotein (BSP) in a dose-dependent manner. The mRNA levels of bone morphogenetic proteins (BMP)-2, BMP-4, collagen type I (COL I) increase. PH increased the proliferation of osteoblasts and directly stimulated ALP and bone matrix proteins (e.g. BSP, BMP-2, BMP-4, COL I, COL II), and these seem to increase trigger osteoblastic differentiation (e.g. mineralized nodule formation). Keywords: Whey protein hydrolysate, MC3T3-E1, Protamex, ALP, osteoblast.