Paper Title
Biocompatibility Of Polycaprolactone Blended Hydroxyapatiteon The Culture Of Human Wharton�s Jelly Mesenchymal Stem Cell Line

Abstract
Polycaprolactone/hydroxyapatite (PCL/HA) composites was used in biomedical implantation technologies for decades because of their proper biocompatible properties. Regenerative therapy using stem cell treatment may need special types of biomaterial as initial scaffold. PCL/HA have never been test for biocompatibility to the human Wharton�s jelly mesenchymal stem cells, WJMSC. The aim of this work is to study the biocompatible of PCL/HA co-polymer on the in vitro culture of WJMSC. PCL/HA was prepared in 2 platforms; 2D-film and 3D-scaffold. Cell attachment efficacy and cell proliferation assay was investigated by using Enzyme-Linked Immunosorbent Assay (ELISA) through the expression level of focal adhesion kinase (FAK) and the expression of the cell cycle regulator protein, cyclin A, respectively. Result showed that the FAK synthesis of cells on 2D-film was enhanced up at greater than 2-times on those of the 3D-scaffold or polystylene (PS) within 21 days of culture. Cell proliferation on the 2 types of composite polymer was found more than 2- times as much in comparison to those on PS, indicating the cell division enhancement. Differentiation and death of the cells was evaluated by using the real-time PCR techniques on the expression of several groups of genes. Stemness was degraded with time indicating the differentiation of the cells in which verified by the expression of the spontaneous differentiation related genes. Death signals was down regulated with time, showing the biocompatibility of the materials and adaptability of WJMSC to the materials. Results from this preliminary work showed the potential of the PCL/HA composite to be utilized as biomaterial for therapeutic purpose using WJMSC. Keywords: MSC, FAK, Cyclin A, Polycaprolactone, Hydroxyapatite, Apoptosis, Necrosis, Differentiation.